Methods: the samples were fixed with 4% formaldehyde, Bouin's fluid, 4% paraformaldehyde and MAW fluid respectively in order to select a suitable fixative for the histamine immunochemical techniques and to define the most appropriate dilution of histamine antibody. 方法:在4%中性甲醛液、Bouin's液、4%多聚甲醛液和MAW液中选择组胺免疫组化技术所适用的固定液,选择组胺抗体的最佳稀释度;
This article collects 446 embryo hands and 409 embryo feet. Their length were measured under stereoscopic microscope after fixing in Bouin's fluid. 本文收集6~11周胚胎手446例,足409例,Bouin液固定后在体视显微镜下测量手、足长度。